Effect of myokine on proliferation and energy metabolism of human breast cancer cell lines

Kazuhiko Higashida, Kentaro Senba,
○Mitsuru Higuchi

 Recent report demonstrated that physical activity probably contributes to a decrease in the risk of breast cancer. The molecular mechanisms underlying this inverse association have not been understood.

The purpose of the present study is to evaluate the involvement of myokines, are secreted by muscle cell in the prevention of breast cancer cell proliferation.

Human breast cancer cell lines (MCF-7, MDA-MB-231, BT-474) were cultured with CTRP15. Forty-eight hours later, The cells were harvested and analyzed for cell proliferation and transporter expression. We found that CTRP15 inhibited the proliferation of the MCF-7 cell line, but not in other two cell lines.  Furthermore, CTRP15 decreased the L-type amino acid transporter protein expression in MCF-7. These results suggest that CTRP15 slowed down MCF-7 proliferation via downregulation of amino acid metabolism.